B16-F10-EGFP cell line

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B16-F10-EGFP Order

B16-F10-EGFP cell line
Catalog#
YC-A011-EGFP-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
B16-F10-EGFP cell line
Order

Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
B16-F10-EGFP
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Mouse
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_XB96(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:B16-F10		 STR Info (Cell bank)
Cell Line:B16-F10
Allele1Allele2Allele3Allele1Allele2Allele3
4-2 237.03
【20.3】		241.13
【21.3】		20.321.3
5-5 343.51
【16】		359.57
【20】		16.020.0
6-4 299.35
【18】		303.47
【19】		18.019.0
6-7 345.99
【15】		15.0
9-2 232.84
【18】		236.94
【19】		18.019.0
12-1 229.14
【17】		233.26
【18】		17.018.0
15-3 200.43
【22.3】		204.54
【23.3】		22.323.3
18-3 147.05
【15】		151.1
【16】		15.016.0
X-1 411.87
【28】		28.0
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases
EGFP validation
B16-F10-EGFP cell line EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the B16-F10 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a B16-F10-EGFP cell line that can maintain long-term, stable and high expression.

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